Pathogen Risk Analysis for Wild Amphibian Populations Following the First Report of a Ranavirus Outbreak in Farmed American Bullfrogs (Lithobates catesbeianus) from Northern Mexico

被引：12

作者：

Saucedo, Bernardo ^{[1
]}

Serrano, Jose M. ^{[2
,3
]}

Jacinto-Maldonado, Monica ^{[4
,5
]}

Leuven, Rob S. E. W. ^{[6
,7
]}

Rocha Garcia, Abraham A. ^{[8
]}

Mendez Bernal, Adriana ^{[8
]}

Grone, Andrea ^{[1
]}

van Beurden, Steven J. ^{[1
]}

Escobedo-Bonilla, Cesar M. ^{[9
]}

机构：

[1] Univ Utrecht, Fac Vet Med, Dept Pathobiol, NL-3584 CL Utrecht, Netherlands

[2] Univ Chile, Fac Ciencias, Dept Ciencias Ecol, Lab Genet & Evoluc, Santiago 3425, Chile

[3] Univ Chile, Fac Med, Programa Fisiol & Biofis, Santiago 8380453, Chile

[4] Univ Nacl Autonoma Mexico, Fac Vet Med, Dept Ethol, Wildlife & Lab Anim, Mexico City 045010, DF, Mexico

[5] Autonomous Univ Mexico, Complex Sci Ctr C3, Mexico City 045010, DF, Mexico

[6] Radboud Univ Nijmegen, Inst Water & Wetland Res, Dept Anim Ecol & Physiol, NL-6500 GL Nijmegen, Netherlands

[7] Netherlands Ctr Expertise Exot Species, NL-6500 GL Nijmegen, Netherlands

[8] Univ Nacl Autonoma Mexico, Fac Vet Med, Dept Pathol 04510, Mexico City 045010, DF, Mexico

[9] Inst Politecn Nacl, CIIDIR Unidad Sinaloa, Dept Aquaculture, Guasave Sinaloa 81101, Mexico

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 01期

关键词：

amphibians; histopathology; immunohistochemistry; Mexico; outbreak; ranavirus; risk assessment; MOLECULAR CHARACTERIZATION; SEQUENCE-ANALYSIS; IRIDOVIRUS; FROGS; CONSERVATION; MORTALITY; INFECTION; VIRUS;

D O I：

10.3390/v11010026

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ranaviruses are the second deadliest pathogens for amphibian populations throughout the world. Despite their wide distribution in America, these viruses have never been reported in Mexico, the country with the fifth highest amphibian diversity in the world. This paper is the first to address an outbreak of ranavirus in captive American bullfrogs (Lithobates catesbeianus) from Sinaloa, Mexico. The farm experienced high mortality in an undetermined number of juveniles and sub-adult bullfrogs. Affected animals displayed clinical signs and gross lesions such as lethargy, edema, skin ulcers, and hemorrhages consistent with ranavirus infection. The main microscopic lesions included mild renal tubular necrosis and moderate congestion in several organs. Immunohistochemical analyses revealed scant infected hepatocytes and renal tubular epithelial cells. Phylogenetic analysis of five partial ranavirus genes showed that the causative agent clustered within the Frog virus 3 clade. Risk assessment with the Pandora(+) protocol demonstrated a high risk for the pathogen to affect amphibians from neighboring regions (overall Pandora risk score: 0.619). Given the risk of American bullfrogs escaping and spreading the disease to wild amphibians, efforts should focus on implementing effective containment strategies and surveillance programs for ranavirus at facilities undertaking intensive farming of amphibians.

引用

页数：15