Identification of thermotolerant Campylobacter species by fluorescence in situ hybridization

被引：10
|
作者：
Poppert, Sven [1 ]
Haas, Michaela [1 ]
Yildiz, Tatjana [1 ]
Alter, Thomas [2 ]
Bartel, Edda [2 ]
Fricke, Ursula [1 ]
Essig, Andreas [1 ]
机构：
[1] Univ Ulm, Inst Med Microbiol & Hyg, Ulm, Germany
[2] Fed Inst Risk Assessment, Berlin, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 06期
关键词：
D O I：
10.1128/JCM.01512-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Thermotolerant Campylobacter spp. ( Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization ( FISH) for the identification of Campylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates of Campylobacter, Arcobacter, and Helicobacter. FISH correctly identified all isolates to the genus level and detected all thermotolerant Campylobacter isolates. The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level.
引用
收藏
页码：2133 / 2136
页数：4
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