An automated sample preparation system for large-scale DNA sequencing

被引：0

作者：

Marziali, A ^{[1
]}

Willis, TD ^{[1
]}

Federspiel, NA ^{[1
]}

Davis, RW ^{[1
]}

机构：

[1] Stanford DNA Sequencing & Technol Ctr, Palo Alto, CA 94304 USA

来源：

GENOME RESEARCH | 1999年 / 9卷 / 05期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day From libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

引用

页码：457 / 462

页数：6

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