STABILITY OF REFERENCE GENES ESTIMATED BY REAL-TIME PCR IN PORCINE LIVER

被引：0
|
作者：
Schmidtova, Anna [1 ]
Knoll, Ales [1 ]
机构：
[1] Mendel Univ Brno, Dept Anim Morphol Physiol & Genet, Zemedelska 1, Brno 61300, Czech Republic
来源：
PROCEEDINGS OF INTERNATIONAL PHD STUDENTS CONFERENCE, (MENDELNET 2016) | 2016年
关键词：
real-time PCR; pig; gene expression; reference gene; ENDOGENOUS CONTROL; EXPRESSION; PIG; TISSUES; MUSCLE;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
It is essential for the method real-time PCR to control variables by validation of normalization of the data. The aim of this study was to develop a set of reference genes for relative quantification of mRNA expression in the porcine liver. The mRNA stability of expression was studied for five genes: GAPDH, HPRT1, PPIA, TOP2B, TBP1, where for each gene the Ct value characterized the level of expression in liver. With the help of geNorm range of stability of analysed genes was (from the most stable to the least): PPIA, TBP1, TOP2B, GAPDH, HPRT1.
引用
收藏
页码：815 / 818
页数：4
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