Characterisation of percentage viability of Streptomyces clavuligerus using image analysis

被引：16
|
作者：
Sebastine, IM [1 ]
Stocks, SM [1 ]
Cox, PW [1 ]
Thomas, CR [1 ]
机构：
[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Birmingham B15 2TT, W Midlands, England
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 06期
关键词：
BacLight; differentiation; fermentation; image analysis; staining; Streptomyces clavuligerus;
D O I：
10.1023/A:1008902330043
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A staining procedure has been developed to identify the different physiological states of Streptomyces clavuligerus using the fluorescent, bacterial viability stain BacLight. An image analysis method was used to quantify the extent of the differently stained regions, and therefore to measure the percentage viability. In a batch fermentation, the viability of the mycelia decreased to 64% during the rapid growth phase. Following a period of fragmentation, the viability was approximately constant at 75% until late in the fermentation, when it increased to 93%.
引用
收藏
页码：419 / 423
页数：5
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