A cocaine hydrolase engineered from human butyrylcholinesterase selectively blocks cocaine toxicity and reinstatement of drug seeking in rats

被引：106
|
作者：
Brimijoin, Stephen [1 ]
Gao, Yang [1 ]
Anker, Justin J. [2 ]
Gliddon, Luke A. [2 ]
LaFleur, David [3 ]
Shah, R. [3 ]
Zhao, Qinghai [3 ]
Singh, M. [3 ]
Carroll, Marilyn E. [2 ]
机构：
[1] Mayo Clin, Dept Mol Pharmacol & Expt Therapeut, Rochester, MN 55905 USA
[2] Univ Minnesota, Dept Psychiat, Minneapolis, MN 55455 USA
[3] CoGenesys Inc, Drug Dev, Rockville, MD USA
来源：
NEUROPSYCHOPHARMACOLOGY | 2008年 / 33卷 / 11期
关键词：
cocaine overdose rescue; addiction relapse; protein-based therapeutics for cocaine abuse; albumin fusion proteins; operant conditioning; drug self-administration rodent model;
D O I：
10.1038/sj.npp.1301666
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Successive rational mutations of human butyrylcholinesterase (BChE) followed by fusion to human serum albumin have yielded an efficient hydrolase that offers realistic options for therapy of cocaine overdose and abuse. This albumin-BChE prevented seizures in rats given a normally lethal cocaine injection (100 mg/kg, i.p.), lowered brain cocaine levels even when administered after the drug, and provided rescue after convulsions commenced. Moreover, it selectively blocked cocaine-induced reinstatement of drug seeking in rats that had previously self-administered cocaine. The enzyme treatment was well tolerated and may be worth exploring for clinical application in humans.
引用
收藏
页码：2715 / 2725
页数：11
相关论文
共 50 条
  • [1] A Cocaine Hydrolase Engineered from Human Butyrylcholinesterase Selectively Blocks Cocaine Toxicity and Reinstatement of Drug Seeking in Rats
    Stephen Brimijoin
    Yang Gao
    Justin J Anker
    Luke A Gliddon
    David LaFleur
    R Shah
    Qinghai Zhao
    M Singh
    Marilyn E Carroll
    Neuropsychopharmacology, 2008, 33 : 2715 - 2725
  • [2] Effects of a cocaine hydrolase engineered from human butyrylcholinesterase on metabolic profile of cocaine in rats
    Chen, Xiabin
    Zheng, Xirong
    Zhou, Ziyuan
    Zhan, Chang-Guo
    Zheng, Fang
    CHEMICO-BIOLOGICAL INTERACTIONS, 2016, 259 : 104 - 109
  • [3] Cocaine Hydrolase Encoded in Viral Vector Blocks the Reinstatement of Cocaine Seeking in Rats for 6 Months
    Anker, Justin J.
    Brimijoin, Stephen
    Gao, Yang
    Geng, Liyi
    Zlebnik, Natalie E.
    Parks, Robin J.
    Carroll, Marilyn E.
    BIOLOGICAL PSYCHIATRY, 2012, 71 (08) : 700 - 705
  • [4] Catalytic activities of a cocaine hydrolase engineered from human butyrylcholinesterase against (+)- and (-)-cocaine
    Xue, Liu
    Hou, Shurong
    Yang, Wenchao
    Fang, Lei
    Zheng, Fang
    Zhan, Chang-Guo
    CHEMICO-BIOLOGICAL INTERACTIONS, 2013, 203 (01) : 57 - 62
  • [5] Preparation and in vivo characterization of a cocaine hydrolase engineered from human butyrylcholinesterase for metabolizing cocaine
    Xue, Liu
    Hou, Shurong
    Tong, Min
    Fang, Lei
    Chen, Xiabin
    Jin, Zhenyu
    Tai, Hsin-Hsiung
    Zheng, Fang
    Zhan, Chang-Guo
    BIOCHEMICAL JOURNAL, 2013, 453 : 447 - 454
  • [6] Cebranopadol Blocks the Escalation of Cocaine Intake and Conditioned Reinstatement of Cocaine Seeking in Rats
    de Guglielmo, Giordano
    Matzeu, Alessandra
    Kononoff, Jenni
    Mattioni, Julia
    Martin-Fardon, Remi
    George, Olivier
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2017, 362 (03): : 378 - 384
  • [7] Kinetic characterization of a cocaine hydrolase engineered from mouse butyrylcholinesterase
    Chen, Xiabin
    Huang, Xiaoqin
    Geng, Liyi
    Xue, Liu
    Hou, Shurong
    Zheng, Xirong
    Brimijoin, Stephen
    Zheng, Fang
    Zhan, Chang-Guo
    BIOCHEMICAL JOURNAL, 2015, 466 : 243 - 251
  • [8] Serotonin depletion attenuates cocaine seeking but enhances sucrose seeking and the effects of cocaine priming on reinstatement of cocaine seeking in rats
    Ly T. Tran-Nguyen
    Jon G. Bellew
    Katherine A. Grote
    Janet L. Neisewander
    Psychopharmacology, 2001, 157 : 340 - 348
  • [9] Serotonin depletion attenuates cocaine seeking but enhances sucrose seeking and the effects of cocaine priming on reinstatement of cocaine seeking in rats
    Tran-Nguyen, LTL
    Bellew, JG
    Grote, KA
    Neisewander, JL
    PSYCHOPHARMACOLOGY, 2001, 157 (04) : 340 - 348
  • [10] PROTECTION AGAINST COCAINE TOXICITY BY HUMAN BUTYRYLCHOLINESTERASE (BCHE) IN RATS
    DRETCHEN, KL
    SINGH, A
    BRADLEY, RM
    LYNCH, TJ
    FASEB JOURNAL, 1992, 6 (04): : A1282 - A1282
12345