MRGD, a MAS-related G-protein Coupled Receptor, Promotes Tumorigenisis and Is Highly Expressed in Lung Cancer

被引：24
|
作者：
Nishimura, Satoko [1 ]
Uno, Makiko [2 ]
Kaneta, Yasuyuki [1 ]
Fukuchi, Keisuke [1 ]
Nishigohri, Haruyuki [1 ]
Hasegawa, Jun [1 ]
Komori, Hironobu [3 ]
Takeda, Shigeki [4 ]
Enomoto, Katsuhiko [5 ]
Nara, Futoshi [6 ]
Agatsuma, Toshinori [1 ]
机构：
[1] Daiichi Sankyo Co Ltd, Biol Res Labs, Shinagawa Ku, Tokyo, Japan
[2] Daiichi Sankyo Co Ltd, Exploratory Res Labs 2, Shinagawa Ku, Tokyo, Japan
[3] Daiichi Sankyo Co Ltd, Oncol Res Labs, Edogawa Ku, Tokyo, Japan
[4] Gunma Univ, Grad Sch Engn, Dept Chem & Chem Biol, Gunma, Japan
[5] Akita Univ, Grad Sch Med, Dept Mol Pathol & Tumor Pathol, Akita 010, Japan
[6] Daiichi Sankyo Co Ltd, Cardiovasc Metabol Res Labs, Shinagawa Ku, Tokyo, Japan
来源：
PLOS ONE | 2012年 / 7卷 / 06期
关键词：
BREAST CARCINOMAS; ES CELLS; C-MYC; ONCOGENE; OVEREXPRESSION; IDENTIFICATION; INVOLVEMENT; PROGNOSIS; SUBSETS; FAMILY;
D O I：
10.1371/journal.pone.0038618
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To elucidate the function of MAS-related GPCR, member D (MRGD) in cancers, we investigated the in vitro and in vivo oncogenic function of MRGD using murine fibroblast cell line NIH3T3 in which MRGD is stably expressed. The expression pattern of MRGD in clinical samples was also analyzed. We found that overexpression of MRGD in NIH3T3 induced focus formation and multi-cellular spheroid formation, and promoted tumors in nude mice. In other words, overexpression of MRGD in NIH3T3 induced the loss of contact inhibition, anchorage-independent growth and in vivo tumorigenesis. Furthermore, it was found that the ligand of MRGD, beta-alanine, enhanced spheroid formation in MRGD-expressing NIH3T3 cells. From investigation of clinical cancer tissues, we found high expression of MRGD in several lung cancers by immunohistochemistry as well as real time PCR. Based on these results, MRGD could be involved in tumorigenesis and could also be a novel anticancer drug target.
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页数：8
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