The J-domain cochaperone Rsp1 interacts with Mto1 to organize noncentrosomal microtubule assembly

被引:7
|
作者
Shen, Juan [1 ,2 ,3 ]
Li, Tianpeng [1 ,2 ,3 ]
Niu, Xiaojia [1 ,2 ,3 ]
Liu, Wenyue [1 ,2 ,3 ]
Zheng, Shengnan [1 ,2 ,3 ]
Wang, Jing [1 ,2 ,3 ]
Wang, Fengsong [4 ]
Cao, Xinwang [4 ]
Yao, Xuebiao [1 ,2 ,3 ]
Zheng, Fan [1 ,2 ,3 ]
Fu, Chuanhai [1 ,2 ,3 ]
机构
[1] Univ Sci & Technol China, Hefei Natl Sci Ctr Phys Sci, Div Mol & Cell Biophys, Hefei 230027, Anhui, Peoples R China
[2] Chinese Acad Sci, Univ Sci & Technol China, Ctr Excellence Mol Cell Sci, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
[3] Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Hefei 230027, Anhui, Peoples R China
[4] Anhui Med Univ, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
GAMMA-TUBULIN COMPLEX; INTERPHASE MICROTUBULES; CELL-DIVISION; PROTEIN; NUCLEATION; CENTROSOMIN; COMPONENT; DYNAMICS; ARRAYS; ACTIVATION;
D O I
10.1091/mbc.E18-05-0279
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microtubule biogenesis initiates at various intracellular sites, including the centrosome, the Golgi apparatus, the nuclear envelope, and preexisting microtubules. Similarly, in the fission yeast Schizosaccharomyces pombe, interphase microtubules are nucleated at the spindle pole body (SPB), the nuclear envelope, and preexisting microtubules, depending on Mto1 activity. Despite the essential role of Mto1 in promoting microtubule nucleation, how distribution of Mto1 in different sites is regulated has remained elusive. Here, we show that the J-domain cochaperone Rsp1 interacts with Mto1 and specifies the localization of Mto1 to non-SPB nucleation sites. The absence of Rsp1 abolishes the localization of Mto1 to non-SPB nucleation sites, with concomitant enrichment of Mto1 to the SPB and the nuclear envelope. In contrast, Rsp1 overexpression impairs the localization of Mto1 to all microtubule organization sites. These findings delineate a previously uncharacterized mechanism in which Rsp1-Mto1 interaction orchestrates non-SPB microtubule formation.
引用
收藏
页码:256 / 267
页数:12
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