Purification and properties of the raw starch-degrading α-amylase of Bacillus sp. IMD 434

被引：51
|
作者：
Hamilton, LM [1 ]
Kelly, CT [1 ]
Fogarty, WM [1 ]
机构：
[1] Natl Univ Ireland Univ Coll Dublin, Dept Ind Microbiol, Dublin 4, Ireland
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 02期
关键词：
Bacillus; alpha-amylase; purification; raw starch-degrading;
D O I：
10.1023/A:1005413816101
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The paw starch-degrading alpha-amylase of Bacillus sp. IMD 434 was purified to homogeneity by acetone precipitation, ion-exchange chromatography and hydrophobic interaction chromatography. The enzyme had a relative molecular mass of 69,200, displayed maximum activity at pH 6.0 and 65 degrees C and released large amounts of glucose and maltose an hydrolysis of starch.
引用
收藏
页码：111 / 115
页数：5
相关论文
共 50 条
  • [1] Purification and properties of the raw starch-degrading α- amylase of Bacillus sp. IMD 434
    Lynn M. Hamilton
    Catherine T. Kelly
    William M. Fogarty
    [J]. Biotechnology Letters, 1999, 21 : 111 - 115
  • [2] The raw starch-degrading alkaline amylase of Bacillus sp IMD 370
    Kelly, CT
    McTigue, MA
    Doyle, EM
    Fogarty, WM
    [J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1995, 15 (05): : 446 - 448
  • [3] Raw starch degradation by the non-raw starch-adsorbing bacterial alpha amylase of Bacillus sp. IMD 434
    Hamilton, LM
    Kelly, CT
    Fogarty, WM
    [J]. CARBOHYDRATE RESEARCH, 1998, 314 (3-4) : 251 - 257
  • [4] Production and properties of the raw starch-digesting α-amylase of Bacillus sp IMD 435
    Hamilton, LM
    Kelly, CT
    Fogarty, WM
    [J]. PROCESS BIOCHEMISTRY, 1999, 35 (1-2) : 27 - 31
  • [5] Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23
    Lin, LL
    Chyau, CC
    Hsu, WH
    [J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1998, 28 : 61 - 68
  • [6] Alkaline amylase of the alkalophilic bacillus sp. IMD 370
    Mc, Tigue, M.A.
    Kelly, C.T.
    Doyle, E.M.
    Fogarty, W.M.
    [J]. Enzyme and Microbial Technology, 1995, 17 (06):
  • [7] Characteristics of raw starch degrading α -amylase from Bacillus aquimaris MKSC 6.2 associated with soft coral Sinularia sp.
    Biochemistry Division, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha 10, Bandung, 40132, Indonesia
    不详
    不详
    [J]. starch, 8 (461-467):
  • [8] Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp. ALSHL3
    Keni Vidilaseris
    Karina Hidayat
    Debbie S. Retnoningrum
    Zeily Nurachman
    Achmad Saefuddin Noer
    Dessy Natalia
    [J]. Biologia, 2009, 64 : 1047 - 1052
  • [9] Characteristics of raw starch degrading α-amylase from Bacillus aquimaris MKSC 6.2 associated with soft coral Sinularia sp.
    Puspasari, Fernita
    Nurachman, Zeily
    Noer, Achmad Saefuddin
    Radjasa, Ocky Karna
    van der Maarel, Marc J. E. C.
    Natalia, Dessy
    [J]. STARCH-STARKE, 2011, 63 (08): : 461 - 467
  • [10] Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimization
    Li, He
    Yao, Dongbang
    Pan, Yan
    Chen, Xin
    Fang, Zemin
    Xiao, Yazhong
    [J]. MICROBIAL CELL FACTORIES, 2022, 21 (01)
12345