Ptychography - a label free, high-contrast imaging technique for live cells using quantitative phase information

被引:121
|
作者
Marrison, Joanne [1 ]
Raety, Lotta [1 ]
Marriott, Poppy [1 ]
O'Toole, Peter [1 ]
机构
[1] Univ York, Dept Biol, Imaging & Cytometry Lab, Biosci Technol Facil, York YO10 5DD, N Yorkshire, England
来源
SCIENTIFIC REPORTS | 2013年 / 3卷
基金
英国生物技术与生命科学研究理事会;
关键词
ANNEXIN-V; MICROSCOPY; CYCLE; TRANSPORT; LENSES;
D O I
10.1038/srep02369
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast, and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis.
引用
收藏
页数:7
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