Identification of Francisella tularensis using real-time fluorescence polymerase chain reaction

被引：6
|
作者：
McAvin, JC [1 ]
Morton, MM [1 ]
Roudabush, RM [1 ]
Atchley, DH [1 ]
Hickman, JR [1 ]
机构：
[1] AF Inst Environm Safety & Occupat Hlth Risk Anal, Mol Epidemiol Branch, Epidemiol Surveillance Div, Brooks AFB, TX 78235 USA
来源：
MILITARY MEDICINE | 2004年 / 169卷 / 04期
关键词：
D O I：
10.7205/MILMED.169.4.330
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A Francisella tularensis-specific, TaqMan probe-based, real-time fluorescence polymerase chain reaction (PCR) assay required approximately 60 minutes and consistently achieved a sensitivity of <= 10 fg of F. tularensis genomic DNA (five genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial organisms representing closely related species, diverse genera, and human genomic DNA resulted in no false positives of significance. The assay was conducted on a field-deployable thermocycler, the R.A.P.I.D. ("Ruggedized" Advanced Pathogen Identification Device), a microbial identification system that can provide rapid and accurate identification F. tularensis.
引用
收藏
页码：330 / 333
页数：4
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