RT-PCR- and MALDI-TOF Mass Spectrometry-Based Identification and Discrimination of Isoforms Homologous to Pufferfish Saxitoxin- and Tetrodotoxin-Binding Protein in the Plasma of Non-Toxic Cultured Pufferfish (Takifugu rubripes)

被引：21

作者：

Tatsuno, Ryohei ^{[1
]}

Yamaguchi, Kenichi ^{[1
,2
,3
]}

Takatani, Tomohiro ^{[3
]}

Arakawa, Osamu ^{[3
]}

机构：

[1] Nagasaki Univ, Grad Sch Sci & Technol, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Fac Fisheries, Div Biochem, Nagasaki 8528521, Japan

[3] Nagasaki Univ, Grad Sch Fisheries Sci & Environm Studies, Nagasaki 8528521, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 01期

关键词：

Takifugu rubripes; tetrodotoxin; toxin-binding protein; (MALDI-TOF) mass spectrometry; FUGU-RUBRIPES; PURIFICATION; GENOME; FISH;

D O I：

10.1271/bbb.120701

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four genes of Takifugu rubripes, tentatively designated Tr1-Tr4, encoding homologs of pufferfish saxitoxin- and tetrodotoxin-binding protein, were identified by BLAST search and 3'-RACE. RT-PCR and MALDI-TOF mass spectrometry allowed the identification and discrimination of Tr isoforms from the non-toxically cultured specimens. The expression of Tr1 and Tr3 mRNAs exclusively in the liver and the presence of their products as 120-kDa plasma proteins were confirmed.

引用

页码：208 / 212

页数：5