A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages

被引：54
|
作者：
Dimitrov, DS
Norwood, D
Stantchev, TS
Feng, YR
Xiao, XD
Broder, CC
机构：
[1] NCI, Lab Expt & Computat Biol, DBS, FCRDC,NIH, Frederick, MD 21702 USA
[2] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD 20814 USA
来源：
VIROLOGY | 1999年 / 259卷 / 01期
关键词：
HIV-1; CD4; CXCR4; coreceptors; chemokine receptors; envelope glycoprotein; membrane fusion;
D O I：
10.1006/viro.1999.9747
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.
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页码：1 / 6
页数：6
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