SPARC Is a Key Regulator of Proliferation, Apoptosis and Invasion in Human Ovarian Cancer

被引:55
|
作者
Chen, Jie [1 ]
Wang, Mei [2 ]
Xi, Bo [1 ]
Xue, Jian [1 ]
He, Dan [1 ]
Zhang, Jie [3 ]
Zhao, Yueran [3 ]
机构
[1] Shandong Univ, Sch Publ Hlth, Dept Maternal & Child Hlth Care, Jinan 250100, Peoples R China
[2] Shandong Univ, Shandong Prov Hosp, Dept Pharm, Jinan 250100, Peoples R China
[3] Shandong Univ, Shandong Prov Hosp, Cent Lab, Jinan 250100, Peoples R China
来源
PLOS ONE | 2012年 / 7卷 / 08期
关键词
PROTEIN EXPRESSION; DOWN-REGULATION; E-CADHERIN; CELL; BREAST; RICH; ACTIVATION; GROWTH; OSTEONECTIN/SPARC; IDENTIFICATION;
D O I
10.1371/journal.pone.0042413
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Secreted protein acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, is implicated in the progression of many cancers. In this study, we investigated the expression and function of SPARC in ovarian cancer. Methods: cDNA microarray analysis was performed to compare gene expression profiles of the highly invasive and the low invasive subclones derived from the SKOV3 human ovarian cancer cell line. Immunohistochemistry (IHC) staining was performed to investigate SPARC expression in a total of 140 ovarian tissue specimens. In functional assays, effects of SPARC knockdown on the biological behavior of ovarian cancer cells were investigated. The mechanisms of SPARC in ovarian cancer proliferation, apoptosis and invasion were also researched. Results: SPARC was overexpressed in the highly invasive subclone compared with the low invasive subclone. High SPARC expression was associated with high stage, low differentiation, lymph node metastasis and poor prognosis of ovarian cancer. Knockdown of SPARC expression significantly suppressed ovarian cancer cell proliferation, induced cell apoptosis and inhibited cell invasion and metastasis. Conclusion: SPARC is overexpressed in highly invasive subclone and ovarian cancer tissues and plays an important role in ovarian cancer growth, apoptosis and metastasis.
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页数:15
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