miR-34a sensitizes lung cancer cells to cisplatin via p53/miR-34a/MYCN axis

被引：41

作者：

Song, Changshan ^{[1
]}

Lu, Pingfang ^{[2
]}

Sun, Guoqiang ^{[2
]}

Yang, Lin ^{[3
]}

Wang, Zhigang ^{[2
]}

Wang, Zheng ^{[3
]}

机构：

[1] Jinan Univ, Affiliated Hosp 1, Dept Thorac Surg, Guangzhou 510630, Guangdong, Peoples R China

[2] Guangdong Med Univ, Affiliated Hosp, Dept Thorac Surg, Zhanjiang 524023, Guangdong, Peoples R China

[3] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Thorac Surg, Shenzhen 518020, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 482卷 / 01期

关键词：

Cisplatin; miR-34a; NSCLC; MYCN; p53; STEM-CELLS; INVASION; APOPTOSIS; GROWTH; DAMAGE; EMT;

D O I：

10.1016/j.bbrc.2016.11.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cisplatin is the most potent and widespread used chemotherapy drug for lung cancer treatment. However, a large proportion of NSCLC patients were insensitive to chemotherapy. This study explored the role of miR-34a in regulating sensitivity of NSCLC cells to cisplatin and its downstream targets. The quantitative PCR result showed that miR-34a expression was upregulated in cisplatin sensitive NSCLC patients compared cisplatin insensitive NSCLC controls. By applying loss-and-gain function analysis, we demonstrated that miR-34a directly targeted to MYCN to sensitize NSCLC cells to cisplatin. In addition, p53 was found to monitor the expression of miR-34a in NSCLC cells after cisplatin treatment. Therefore, the sensitivity of cisplatin in NSCLC cells was modulated via p53/miR-34a/MYCN axis. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：22 / 27

页数：6

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