The adhesion and proliferation of bone marrow-derived mesenchymal stem cells promoted by nanoparticle surface

This study's aim consists of evaluating the adhesion and proliferation of mesenchymal stem cells (MSCs) derived from rat bone marrow on nanoparticle Titanium (Ti) surface. Hence, passage 3 MSCs were, respectively, seeded on nanoparticle Ti and pure Ti surfaces and then cultured for 32 h. Cell morphology and viability were separately examined by scanning electron microscopy and 3-(4,5-dimethylthiazsol-2-yl)-2,5-diphenyltetrazolium bromide assay. Moreover, the mitotic rate of the attached MSCs was observed through immunocytochemistry. The real-time polymerase chain reaction was applied to determine the adhesion-associated messenger ribonucleic acid (mRNA), CD44 gene encoding variant isoform 6 (CD44 V6), and the integrin beta 1 level. The results showed that MSCs performed better in faster extension on the nanoparticle Ti surface than on the pure Ti surface after culturing for 4 h, and were quicker in fusion patterns after 16 h. Furthermore, cell viability was significantly increased on the nanoparticle Ti surface compared to that of the pure Ti surface 16 h after initial seeding (p < 0.05), and the mitotic rate of attached MSCs on the nanoparticle Ti surface was higher than that on the pure Ti surface after 32 h (p < 0.05). More interestingly, the CD44 V6 and integrin beta 1 mRNA in the nanoparticle Ti surface group expressed higher than that in the pure Ti surface group after 4 h (p < 0.05), and positive correlation between CD44 V6 and integrin beta 1 was found through statistical analysis (correlation coefficient r(s) = 0.98, p < 0.05). Our study's result indicates that a nanoparticle Ti surface can significantly promote the adhesion and proliferation of MSCs, and also improve the bioactivity of Ti surface.