Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells

被引:11
|
作者
Boeker, Kai Oliver [1 ]
Kleinwort, Frederick [2 ]
Klein-Wiele, Jan-Hendrick [2 ]
Simon, Peter [2 ]
Jaeckle, Katharina [1 ]
Taheri, Shahed [1 ]
Lehmann, Wolfgang [1 ]
Schilling, Arndt F. [1 ]
机构
[1] Univ Med Ctr Goettingen, Dept Trauma Surg Orthopaed & Plast Surg, Robert Koch Str 40, D-37075 Gottingen, Germany
[2] Laser Lab Gottingen eV LLG, Hans Adolf Krebs Weg 1, D-37077 Gottingen, Germany
关键词
titanium; steel; implant; laser beam interference ablation; surface nano-topography; nano-topology; stem cell; bone; osteoblast; periodic nano-structures; PATTERN GENERATION; GRATING STRUCTURES; GENE-EXPRESSION; FABRICATION; FRACTURES; REMOVAL; SEARCH; METALS; MATRIX;
D O I
10.3390/ma13163526
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Metal implants used in trauma surgeries are sometimes difficult to remove after the completion of the healing process due to the strong integration with the bone tissue. Periodic surface micro- and nanostructures can directly influence cell adhesion and differentiation on metallic implant materials. However, the fabrication of such structures with classical lithographic methods is too slow and cost-intensive to be of practical relevance. Therefore, we used laser beam interference ablation structuring to systematically generate periodic nanostructures on titanium and steel plates. The newly developed laser process uses a special grating interferometer in combination with an industrial laser scanner and ultrashort pulse laser source, allowing for fast, precise, and cost-effective modification of metal surfaces in a single step process. A total of 30 different periodic topologies reaching from linear over crossed to complex crossed nanostructures with varying depths were generated on steel and titanium plates and tested in bone cell culture. Reduced cell adhesion was found for four different structure types, while cell morphology was influenced by two different structures. Furthermore, we observed impaired osteogenic differentiation for three structures, indicating reduced bone formation around the implant. This efficient way of surface structuring in combination with new insights about its influence on bone cells could lead to newly designed implant surfaces for trauma surgeries with reduced adhesion, resulting in faster removal times, reduced operation times, and reduced complication rates.
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页数:16
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