Four domestic winter wheat varieties (Superzitarka, Zitarka, Monika and Srpanjka) were grown under field conditions (Osijek hydromeliorated hypoglay) in the period 2001-2004. Presowing N (PS-N) was applied in four rates (40, 70, 100 and 130 kg N ha(-1)). Top-dressing N (TD-N) was applied in the amounts (kg N ha(-1)) 0 (without TD), 40 (TD in tillering), 95 (tittering + stem elongation) and 120 (tittering + stem elongation + before shutting). Differences of yields among the years were in the ranges from 7.50 to 8.40 t ha(-1) and proteins from 13.4% to 14.2%. Yield differences among the varieties (4-year means) were in the range from 5.57 t ha(-1) to 9.40 t ha(-1). Srpanjka had significantly the highest yield for all four tested years (mean 9.40 t ha(-1)), while yield of the last-ranged Monika was for 41% lower (mean 5.57 t ha(-1)). However, the highest yield of Srpanjka had significant lowest grain protein (means 13.4% and 13.8%, for Srpanjka and remaining three varieties). PS-N resulted by non-significant yield differences among the treatments (4-year means in range from 7.91 to 7.99 t ha(-1)). Also, wheat yields were independent on TD-N fertilization (4-year means 7.97 and 8.00 t ha(-1)). We presume that high fertility of the soil could be responsible for low response of wheat to N. However, for two growing seasons (2001 and 2002) application of the highest PS-N rate influenced significantly on increase of grain protein compared to the lowest N rate. Especially high influences were found for 2001 (13.1 % and 13.9% protein, for 40 and 130 kg N ha(-1), respectively). TD-N influenced also on protein contents (13.5 and 14.0%, for 0 and 120 kg N ha(-1), respectively).