The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase

被引：5
|
作者：
Li, Xueliang [1 ]
Chen, Xiaoxu [1 ]
Liu, Yingdong [1 ]
Zhang, Pengfei [1 ]
Zheng, Yi [1 ]
Zeng, Wenxian [1 ]
机构：
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling, Shaanxi, Peoples R China
来源：
FRONTIERS IN GENETICS | 2020年 / 11卷
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
SETDB1; NOX4; ROS; spermatogonial stem cell; FOXO TRANSCRIPTION FACTORS; OXIDATIVE STRESS; REACTIVE OXYGEN; UP-REGULATION; NOX4; APOPTOSIS; ROS; ACTIVATION; EXPRESSION; DAF-16;
D O I：
10.3389/fgene.2020.00997
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
SETDB1, a histone H3 lysine 9 (H3K9) methyltransferase, is crucial in meiosis and embryo development. This study aimed to investigate whether SETDB1 was associated with spermatogonial stem cells (SSC) homeostasis. We found that knockdown ofSetdb1impaired cell proliferation, led to an increase in reactive oxygen species (ROS) level through NADPH oxidase, andSetdb1deficiency activated ROS downstream signaling pathways, including JNK and p38 MAPK, which possibly contributed to SSC apoptosis. Melatonin scavenged ROS and rescued the phenotype ofSetdb1KD. In addition, we demonstrated that SETDB1 regulated NADPH oxidase 4 (Nox4) andE2F1. Therefore, this study uncovers the new roles of SETDB1 in mediating intracellular ROS homeostasis for the survival of SSC.
引用
收藏
页数：12
相关论文
共 50 条
  • [1] Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway
    Liu, Tiantian
    Chen, Xiaoxu
    Li, Tianjiao
    Li, Xueliang
    Lyu, Yinghua
    Fan, Xiaoteng
    Zhang, Pengfei
    Zeng, Wenxian
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2017, 1860 (10): : 1094 - 1102
  • [2] HISTONE METHYLTRANSFERASE SETDB1 REGULATES ENERGY METABOLISM IN HEMATOPOIETIC STEM AND PROGENITOR CELLS
    Koide, Shuhei
    Takubo, Keiyo
    Oshima, Motohiko
    Miyagi, Satoru
    Saraya, Atsunori
    Wang, Changshan
    Matsui, Hirotaka
    Kimura, Hiroshi
    Shinkai, Yoichi
    Suda, Toshio
    Iwama, Atsushi
    EXPERIMENTAL HEMATOLOGY, 2015, 43 (09) : S73 - S73
  • [3] The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice
    J An
    X Zhang
    J Qin
    Y Wan
    Y Hu
    T Liu
    J Li
    W Dong
    E Du
    C Pan
    W Zeng
    Cell Death & Disease, 2014, 5 : e1196 - e1196
  • [4] The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice
    An, J.
    Zhang, X.
    Qin, J.
    Wan, Y.
    Hu, Y.
    Liu, T.
    Li, J.
    Dong, W.
    Du, E.
    Pan, C.
    Zeng, W.
    CELL DEATH & DISEASE, 2014, 5 : e1196 - e1196
  • [5] The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells
    Rapone, Roberta
    Del Maestro, Laurence
    Bouyioukos, Costas
    Albini, Sonia
    Cruz-Tapias, Paola
    Joliot, Veronique
    Cosson, Bertrand
    Ait-Si-Ali, Slimane
    ISCIENCE, 2023, 26 (08)
  • [6] The histone methyltransferase SETDB1 contributes to melanoma tumorigenesis
    Orouji, E.
    Larribere, L.
    Novak, D.
    Schoeler, N.
    Bernhardt, M.
    Weina, K.
    Galach, M.
    Reith, M.
    Gebhardt, C.
    Umansky, V.
    Utikal, J.
    JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT, 2014, 12 : 17 - 17
  • [7] Analysis of the subcellular localization of the human histone methyltransferase SETDB1
    Tachibana, Keisuke
    Gotoh, Eiko
    Kawamata, Natsuko
    Ishimoto, Kenji
    Uchihara, Yoshie
    Iwanari, Hiroko
    Sugiyama, Akira
    Kawamura, Takeshi
    Mochizuki, Yasuhiro
    Tanaka, Toshiya
    Sakai, Juro
    Hamakubo, Takao
    Kodama, Tatsuhiko
    Doi, Takefumi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2015, 465 (04) : 725 - 731
  • [8] Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma
    Ho, Yi-Jung
    Lin, Yueh-Min
    Huang, Yen-Chi
    Chang, Jungshan
    Yeh, Kun-Tu
    Lin, Liang-In
    Gong, Zhiyuan
    Tzeng, Tsai-Yu
    Lu, Jeng-Wei
    APMIS, 2017, 125 (11) : 985 - 995
  • [9] Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma
    Tzeng, Tsai-Yu
    CANCER SCIENCE, 2018, 109 : 1242 - 1242
  • [10] Histone methyltransferase Setdb1 is indispensable for Meckel's cartilage development
    Yahiro, Kohei
    Higashihori, Norihisa
    Moriyama, Keiji
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 482 (04) : 883 - 888
12345