Effect of porosity on long-term degradation of poly (ε-caprolactone) scaffolds and their cellular response

被引:74
|
作者
Zhang, Qingchun [1 ]
Jiang, Yun [2 ]
Zhang, Yan [1 ]
Ye, Zhaoyang [3 ]
Tan, Wensong [3 ]
Lang, Meidong [1 ]
机构
[1] E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China
[2] Lib Zhejiang Univ Technol, Hangzhou 310023, Zhejiang, Peoples R China
[3] E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
基金
中国国家自然科学基金;
关键词
Poly (epsilon-caprolactone); Scaffold; Degradation; Porosity; Cell culture; IN-VITRO DEGRADATION; PCL-TCP SCAFFOLDS; VIVO DEGRADATION; PORE-SIZE; BONE; FABRICATION; BEHAVIOR; POROGEN; PROFILE;
D O I
10.1016/j.polymdegradstab.2012.10.008
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Porous poly(epsilon-caprolactone) (PCL) scaffolds are widely used as in vivo implants in tissue engineering, and their long-term degradation behaviors are of great importance for their in vivo performances. However, the influence of porosity on long-term degradation of PCL scaffold in phosphate buffer solution (PBS) has been rarely reported so far. Herein, a 72-week degradation study of PCL scaffolds with various porosities was conducted to elucidate the changes of physico-chemical properties such as weight, molecular weight, morphology and compressive modulus. Within 72 weeks, PCL scaffolds experienced three stages: stable stage, mechanical loss stage and structural collapse stage. The higher porosity induced the severer loss of weight, molecular weight and compressive modulus. It was found that a minimal acid autocatalysis also happened in the scaffold samples with low porosities (less than 85%). Cellular response on the scaffolds with various porosities was further evaluated. The cell ingrowth improved on the scaffold with high porosity (e.g. S-10) in contrast to those with low porosity (e.g. S-6 and S-4). The combined results demonstrated that an optimal porosity of PCL scaffolds should be designed greater than 90% due to the appropriate degradation rate and good cell performance. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:209 / 218
页数:10
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