Somatic Embryogenesis and Shoot Development in Rosa hybrida L.

This study was conducted to induce somatic embryogenesis in in vitro root and leaf explants of different Rosa hybrid cultivars. Somatic embryogenesis was induced at the ratio of 2.9 similar to 20.5% from in vitro roots of Rosa hybrida 'Charming', 'Sweet Yellow' (Korean cultivar), and 'Tineke' cultured in SH medium supplemented with 5 or 11 mg.L-1 2,4-D for 2 or 4 similar to 8 weeks followed by being culturing on SH medium supplemented with 3 mg.L-1 2,4-D for 1 similar to 6 weeks. It was confirmed that 'Sweet Yellow' was a very promising genotype in terms of getting embryos from embryogenic callus within 1 similar to 2 weeks. Embryogenic calli with embryo of 'Tineke' were changed into cotyledonary-stage embryos in SH medium supplemented with 3 mg.L-1 2,4-D without changing the medium. Shoots generated from cotyledonary-stage embryos of 'Tineke' cultured in MS medium supplemented with 2 mg.L-1 glycine and 100 mg.L-1 myo-inositol. Furthermore, somatic embryogenesis and organogenesis were induced from in vitro leaf explants in MS medium supplemented with 2.5 mg.L-1 2,4-D for 4 weeks without light followed by culture in 1/2 MS medium supplemented with different plant growth regulators at a sort or its concentration. The effect of plant growth regulators on embryogenesis from calli using the leaf showed different reactions depending on the genotypes.