TRIP12 functions as an E3 ubiquitin ligase of APP-BP1

被引:28
|
作者
Park, Yoon [1 ]
Yoon, Sungjoo Kim [2 ]
Yoon, Jong-Bok [1 ]
机构
[1] Yonsei Univ, Dept Biochem, Seoul 120749, South Korea
[2] Catholic Univ, Dept Biomed Sci, Seoul 137040, South Korea
关键词
APP-BP1; TRIP12; NEDD8; E3 ubiquitin ligase; protein degradation;
D O I
10.1016/j.bbrc.2008.07.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The NEDD8 pathway plays an essential role in various physiological processes, Such as cell cycle progression and signal transduction. The conjugation of NEDD8 to target proteins is initiated by the NEDD8-activating enzyme composed of APP-BP1 and Uba3. In the present study, we show that APP-BP1 is degraded by ubiquitin-dependent proteolysis. To study biological functions of TRIP12, a HECT domain-containing E3 ubiquitin ligase, we used the yeast two-hybrid system and identified APP-BP1 as its binding partner. Immunoprecipitation analysis showed that TRIP12 specifically interacts with the APP-BP1 monomer but not with the APP-BP1/Uba3 heterodimer. Overexpression of TRIP12 enhanced the degradation of APP-BP1, whereas knockdown of TRIP12 stabilized it. in vitro ubiquitination assays revealed that TRIP12 functions as an E3 enzyme of APP-BP1 and additionally requires an E4 activity for polyubiquitination of APP-BP1. Moreover, neddylation of endogenous CUL1 was increased in TRIP12 knockdown cells, while complementation of the knockdown cells with TRIP12 lowered neddylated CUL1. Our data suggest that that TRIP12 promotes degradation of APP-BP1 by catalyzing its ubiquitination, which in turn modulates the neddylation pathway. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:294 / 298
页数:5
相关论文
共 50 条
  • [1] E3 Ubiquitin Ligase TRIP12: Regulation, Structure, and Physiopathological Functions
    Brunet, Manon
    Vargas, Claire
    Larrieu, Dorian
    Torrisani, Jerome
    Dufresne, Marlene
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (22) : 1 - 34
  • [2] The E3 ubiquitin ligase TRIP12, a new mitotic protein
    Larrieu, D.
    Brunet, M.
    Hanoun, N.
    Ligat, L.
    Dagnon, L.
    Lulka, H.
    Pommier, R. M.
    Selves, J.
    Jady, B.
    Bartholin, L.
    Cordelier, P.
    Dufresne, M.
    Torrisani, J.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2018, 29 (26) : 195 - 196
  • [3] The E3 Ubiquitin Ligase Activity of Trip12 Is Essential for Mouse Embryogenesis
    Kajiro, Masashi
    Tsuchiya, Mai
    Kawabe, Yoh-ichi
    Furumai, Ryohei
    Iwasaki, Naoya
    Hayashi, Yuki
    Katano, Miyuki
    Nakajima, Yuka
    Goto, Natsuka
    Watanabe, Tatsuya
    Murayama, Akiko
    Oishi, Hisashi
    Ema, Masatsugu
    Takahashi, Satoru
    Kishimoto, Hiroyuki
    Yanagisawa, Junn
    [J]. PLOS ONE, 2011, 6 (10):
  • [4] The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability
    Larrieu, D.
    Brunet, M.
    Vargas, C.
    Hanoun, N.
    Ligat, L.
    Dagnon, L.
    Lulka, H.
    Pommier, R. M.
    Selves, J.
    Jady, B. E.
    Bartholin, L.
    Cordelier, P.
    Dufresne, M.
    Torrisani, J.
    [J]. SCIENTIFIC REPORTS, 2020, 10 (01)
  • [5] Control of TGFβ signalling by ubiquitination independent function of E3 ubiquitin ligase TRIP12
    Kripa S Keyan
    Safa Salim
    Swetha Gowda
    Doua Abdelrahman
    Syeda Sakina Amir
    Zeyaul Islam
    Claire Vargas
    Maria Teresa Bengoechea-Alonso
    Amira Alwa
    Subrat Dahal
    Prasanna R. Kolatkar
    Sahar Da’as
    Jerome Torrisani
    Johan Ericsson
    Farhan Mohammad
    Omar M Khan
    [J]. Cell Death & Disease, 14
  • [6] Control of TGFβ signalling by ubiquitination independent function of E3 ubiquitin ligase TRIP12
    Keyan, Kripa S.
    Salim, Safa
    Gowda, Swetha
    Abdelrahman, Doua
    Amir, Syeda Sakina
    Islam, Zeyaul
    Vargas, Claire
    Bengoechea-Alonso, Maria Teresa
    Alwa, Amira
    Dahal, Subrat
    Kolatkar, Prasanna R.
    Da'as, Sahar
    Torrisani, Jerome
    Ericsson, Johan
    Mohammad, Farhan
    Khan, Omar M.
    [J]. CELL DEATH & DISEASE, 2023, 14 (10)
  • [7] The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability
    D. Larrieu
    M. Brunet
    C. Vargas
    N. Hanoun
    L. Ligat
    L. Dagnon
    H. Lulka
    R. M. Pommier
    J. Selves
    B. E. Jády
    L. Bartholin
    P. Cordelier
    M. Dufresne
    J. Torrisani
    [J]. Scientific Reports, 10
  • [8] The E3 ubiquitin ligase TRIP12 is required for pancreatic acinar cell plasticity and pancreatic carcinogenesis
    Brunet, Manon
    Vargas, Claire
    Fanjul, Marjorie
    Varry, Damien
    Hanoun, Naima
    Larrieu, Dorian
    Pieruccioni, Laetitia
    Labrousse, Guillaume
    Lulka, Hubert
    Capilla, Florence
    Ricard, Alban
    Selves, Janick
    Couvelard, Anne
    Gigoux, Veronique
    Cordelier, Pierre
    Guillermet-Guibert, Julie
    Dufresne, Marlene
    Torrisani, Jerome
    [J]. JOURNAL OF PATHOLOGY, 2024, 263 (4-5): : 466 - 481
  • [9] Trip12 is an E3 ubiquitin ligase for USP7/HAUSP involved in the DNA damage response
    Liu, Xiaoliang
    Yang, Xiangcai
    Li, Yongxin
    Zhao, Shuhua
    Li, Chaocui
    Ma, Pengcheng
    Mao, Bingyu
    [J]. FEBS LETTERS, 2016, 590 (23) : 4213 - 4222
  • [10] E3 Ubiquitin Ligase TRIP12 Controls Exit from Mitosis via Positive Regulation of MCL-1 in Response to Taxol
    Keyan, Kripa S.
    Alanany, Rania
    Kohil, Amira
    Khan, Omar M.
    [J]. CANCERS, 2023, 15 (02)