Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum

被引：0
|
作者：
Ribon, AOB
Coelho, JLC
de Barros, EG
Araújo, EF [1 ]
机构：
[1] Univ Fed Vicosa, Dept Microbiol, Vicosa, MG, Brazil
[2] Univ Fed Vicosa, Dept Biol Geral, BR-36571000 Vicosa, MG, Brazil
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 05期
关键词：
filamentous fungi; gene cloning; pectinase; sequence homology;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.
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页码：395 / 399
页数：5
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