Manganese distribution in olfactory bulbs of rat measured by synchrotron radiation x-ray fluorescence

Mn2+-enhanced magnetic resonance imaging is very useful for mapping the olfactory bulb, but has poor positional resolution ( similar to 100 mum) and difficulty with the quantitative analysis of Mn. Synchrotron Radiation X-ray Fluorescence ( beam size similar to 20 mum, detect on limit of Mn similar to 10(-11) g) and Energy Disperse X-ray Fluorescence combined with multi-variable statistics were employed to investigate the positional distribution of manganese and other elements in slices of the olfactory bulk of SD rats. The naris of rats were pipetted with MnCl2 ( Mn group n = 4) compared with the control group ( n = 3). Our results show that the manganese distributions in the slices of both groups were non-homogeneous. The difference in the contents of Mn of rats treated with MnCl2 is as high as 50 times. Rats treated with MnCl2 exhibited a strong correlation (0.985, p = 0.000) between the positional distribution of Mn and Ca, and the contents of Mn, Ca, Zn and Br of the Mn group were 55%, 82%, 42% and 72% higher than the control group.