Detecting membrane lipid microdomains by two-photon fluorescence microscopy

被引：7
|
作者：
Parasassi, T [1 ]
Gratton, E [1 ]
Zajicek, H [1 ]
Levi, M [1 ]
Yu, WM [1 ]
机构：
[1] CNR, Ist Med Sperimentale, I-00137 Rome, Italy
来源：
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE | 1999年 / 18卷 / 05期
关键词：
D O I：
10.1109/51.790991
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Microscopy images of the generalized polarization (GP) of the lipid probe 2-dimethylamino-6-lauroylnapthalene (LAURDAN) in phospholipid vesicles and in natural membranes were obtained by using two-photon excitation. The images show different distribution of the GP value depending on membrane composition. The use of linearly polarized excitation allowed the attribution of the GP heterogeneity to coexisting membrane domains of different dynamic properties. Based on the photoselection operated by the excitation polarization, a model is proposed to explain the results and to estimate the domains' dimension.
引用
收藏
页码：92 / 99
页数：8
相关论文
共 50 条
  • [1] Detecting membrane lipid microdomains by two-photon fluorescence microscopy
    Parasassi, Tiziana
    Gratton, Enrico
    Zajicek, Hubert
    Levi, Moshe
    Yu, Weiming
    IEEE Engineering in Medicine and Biology Magazine, 18 (05): : 92 - 99
  • [2] Two-photon excitation fluorescence microscopy
    So, PTC
    Dong, CY
    Masters, BR
    Berland, KM
    ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 : 399 - 429
  • [3] Adaptive optics two-photon fluorescence microscopy
    Zhou, Yaopeng
    Bifano, Thomas
    Lin, Charles
    MEMS ADAPTIVE OPTICS, 2007, 6467
  • [4] Two-photon fluorescence surface wave microscopy
    Goh, JYL
    Somekh, MG
    See, CW
    Pitter, MC
    Vere, KA
    O'Shea, P
    JOURNAL OF MICROSCOPY, 2005, 220 : 168 - 175
  • [5] New trends in two-photon fluorescence microscopy
    So, PTC
    BIOPHYSICAL JOURNAL, 1998, 74 (02) : A8 - A8
  • [6] Detecting reactive oxygen species in skin using two-photon fluorescence imaging microscopy
    Hanson, KM
    Hayden, PJ
    Kubilus, J
    Clegg, RM
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2004, 122 (03) : A140 - A140
  • [7] Visualization and properties of lipid phases in giant unilamellar vesicles by two-photon fluorescence microscopy
    Celli, A
    Beretta, S
    Gratton, E
    BIOPHYSICAL JOURNAL, 2002, 82 (01) : 428A - 428A
  • [8] Lipid-protein interactions revealed by two-photon microscopy and fluorescence correlation spectroscopy
    Sánchez, SA
    Gratton, E
    ACCOUNTS OF CHEMICAL RESEARCH, 2005, 38 (06) : 469 - 477
  • [9] A comparison study of detecting gold nanorods in living cells with confocal reflectance microscopy and two-photon fluorescence microscopy
    Zhou, Y.
    Wu, X.
    Wang, T.
    Ming, T.
    Wang, P. N.
    Zhou, L. W.
    Chen, J. Y.
    JOURNAL OF MICROSCOPY, 2010, 237 (02) : 200 - 207
  • [10] Two-photon fluorescence microscopy with differential aberration imaging
    Chu, Kengyeh K.
    Leray, Aymeric
    Bifano, Thomas G.
    Mertz, Jerome
    MEMS ADAPTIVE OPTICS III, 2009, 7209
12345