P15® INDUCES RUNX2 IN BONE MARROW DERIVED STEM CELLS

被引：0
|
作者：
Lauritano, D. [1 ]
Carinci, F. [2 ]
Zollino, I. [2 ]
Hassanipour, A. [2 ]
Saggese, V. [1 ]
Palmieri, A. [2 ]
Girardi, A. [3 ]
Cura, F. [3 ]
Piras, A. [4 ]
Zamboni, P. [5 ]
Brunelli, G. [4 ,6 ]
机构：
[1] Univ Milano Bicocca, Dept Neurosci & Biomed Technol, Milan, Italy
[2] Univ Ferrara, Dept DMCCC, Sect Maxillofacial & Plast Surg, I-44100 Ferrara, Italy
[3] Univ Bologna, Ctr Mol Genet, Dept Histol Embryol & Appl Biol, CARISBO Fdn, Bologna, Italy
[4] Univ Cagliari, Dent Clin, I-09124 Cagliari, Italy
[5] Univ Ferrara, Dept Radiol Anesthesiol & Surg, I-44100 Ferrara, Italy
[6] Don Orione Inst, Dept Dent & Maxillofacial & Plast Surg, Bergamo, Italy
来源：
EUROPEAN JOURNAL OF INFLAMMATION | 2012年 / 10卷 / 01期
关键词：
stem cell; biomaterial; P-15; differentiation; bone; IN-VITRO; ATTACHMENT; DEFECTS; P-15;
D O I：
暂无
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Peptide-15 (P-15) is. P-15 an analog of the cell binding domain of collagen, has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. In this study we investigated the osteo-inductives properties of P15 on stem cells derived from bone marrow. In Real Time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR) we quantified the mRNA expression of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG). Gene expression in BMSC was then compared with the gene expression in Human Osteoblasts (HOb) treated with P15, to evaluate the potential effect of this biomaterial in osteoblasts differentiation.
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页码：95 / 100
页数：6
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