Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

被引:2
|
作者
Chen, Lin [1 ]
Leng, Changlong [2 ]
Ru, Qin [2 ]
Xiong, Qi [2 ]
Zhou, Mei [2 ]
Wu, Yuxiang [1 ]
机构
[1] Jianghan Univ, Sch Phys Educ, Wuhan 430056, Peoples R China
[2] Jianghan Univ, Wuhan Inst Biomed Sci, Wuhan 430056, Peoples R China
基金
中国国家自然科学基金;
关键词
DORSAL-ROOT GANGLION; ATP-ACTIVATED CURRENT; P2X(3) RECEPTOR; SENSORY NEURONS; PURINERGIC MECHANISMS; SPINAL MICROGLIA; DOWN-REGULATION; EXPRESSION; SUBUNITS; PATHOPHYSIOLOGY;
D O I
10.1155/2020/9861459
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The distributions of P2X subtypes during peripheral neuropathic pain conditions and their differential roles are not fully understood. To explore these characteristics, the lumbosacral dorsal root ganglion (DRG) in the chronic constriction injury (CCI) sciatic nerve rat model was studied. Retrograde trace labeling combined with immunofluorescence technology was applied to analyze the distribution of neuropathic nociceptive P2X1-6 receptors. Our results suggest that Fluoro-Gold (FG) retrograde trace labeling is an efficient method for studying lumbosacral DRG neurons in the CCI rat model, especially when the DRG neurons are divided into small, medium, and large subgroups. We found that neuropathic nociceptive lumbosacral DRG neurons (i.e., FG-positive cells) were significantly increased in medium DRG neurons, while they declined in the large DRG neurons in the CCI group. P2X3 receptors were markedly upregulated in medium while P2X2 receptors were significantly decreased in small FG-positive DRG neurons. There were no significant changes in other P2X receptors (including P2X1, P2X4, P2X5, and P2X6). We anticipate that P2X receptors modulate nociceptive sensitivity primarily through P2X3 subtypes that are upregulated in medium neuropathic nociceptive DRG neurons and/or via the downregulation of P2X2 cells in neuropathic nociceptive small DRG neurons.
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页数:15
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