Effects of methyl jasmonate and salicylic acid on cell growth and cryptotanshinone formation in Ti transformed Salvia miltiorrhiza cell suspension cultures

被引：28
|
作者：
Chen, H [1 ]
Chen, F [1 ]
机构：
[1] Univ Hong Kong, Dept Bot, Hong Kong, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 09期
关键词：
cryptotanshinone; ibuprofen; methyl jasmonate; salicylic acid; Salvia miltiorrhiza;
D O I：
10.1023/A:1005551911450
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Exogenous methyl jasmonate (MJ) or salicylic acid (SA) when applied alone failed to induce cryptotanshinone (a phytoalexin) formation in Ti-transformed Salvia miltiorrhiza cell suspension cultures. However, when applied in combination with yeast elicitor, SA at 50-500 mu M enhanced the yeast elicitor-induced cryptotanshinone formation while MJ reduced the yeast elicitor-induced cryptotanshinone formation. Ibuprofen at 100 mu M did not inhibit the yeast elicitor-induced cryptotanshinone formation. DMSO was superior to ethanol as a solvent for introducing MJ to the cell cultures.
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页码：803 / 807
页数：5
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