Use and Evaluation of Molecular Diagnostics for Pneumonia Etiology Studies

被引:23
|
作者
Bhat, Niranjan [1 ,3 ]
O'Brien, Katherine L. [2 ]
Karron, Ruth A. [1 ]
Driscoll, Amanda J. [2 ]
Murdoch, David R. [4 ,5 ]
机构
[1] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Int Hlth, Ctr Immunizat Res, Baltimore, MD 21205 USA
[2] Johns Hopkins Bloomberg Sch Publ Hlth, Int Vaccine Access Ctr, Baltimore, MD 21205 USA
[3] Johns Hopkins Sch Med, Dept Pediat, Div Infect Dis, Baltimore, MD USA
[4] Canterbury Hlth Labs, Microbiol Unit, Christchurch, New Zealand
[5] Univ Otago, Dept Pathol, Christchurch, New Zealand
基金
比尔及梅琳达.盖茨基金会;
关键词
RESPIRATORY-TRACT INFECTIONS; REAL-TIME PCR; ACID AMPLIFICATION ASSAYS; MULTIPLEX PCR; DIFFERENTIAL DETECTION; OLIGONUCLEOTIDE ARRAY; ENZYME HYBRIDIZATION; HIGH-THROUGHPUT; VIRUSES; SYSTEM;
D O I
10.1093/cid/cir1060
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Comprehensive microbiological testing will be a core function of the Pneumonia Etiology Research for Child Health (PERCH) project. The development stage of PERCH provided the time and resources necessary for us to conduct a comprehensive review of the current state of respiratory diagnostics. These efforts allowed us to articulate the unique requirements of PERCH, establish that molecular methods would be central to our testing strategy, and focus on a short list of candidate platforms. This process also highlighted critical challenges in the general design and interpretation of diagnostic evaluation studies, particularly in the field of respiratory infections. Although our final molecular diagnostic platform was ultimately selected on the basis of operational and strategic considerations determined by the specific context of PERCH, our review highlighted several conceptual and practical challenges in respiratory diagnostics that have broader relevance for the performance and interpretation of pneumonia research studies.
引用
收藏
页码:S153 / S158
页数:6
相关论文
共 50 条
  • [1] Molecular diagnostics in pneumonia
    McDunn, J
    PROCEEDINGS OF THE 11TH CONGRESS OF THE EUROPEAN SHOCK SOCIETY, 2005, : 147 - 149
  • [2] Molecular diagnostics in pneumonia.
    McDunn, JE
    Husain, KD
    Coopersmith, C
    Dunne, M
    Townsend, RR
    Cobb, JP
    SHOCK, 2005, 23 : 2 - 3
  • [3] Molecular diagnostics of atypical pneumonia
    Yi-Wei TANG Departments of Medicine and Pathology
    Acta Pharmacologica Sinica, 2003, (12) : 134 - 139
  • [4] Bacterial and viral etiology in hospitalized community acquired pneumonia with molecular methods and clinical evaluation
    Serin, Derya Caglayan
    Pullukcu, Husnu
    Cicek, Candan
    Sipahi, Oguz Resat
    Tasbakan, Sezai
    Atalay, Sabri
    JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, 2014, 8 (04): : 510 - 518
  • [5] The Role of Postmortem Studies in Pneumonia Etiology Research
    Turner, Gareth D. H.
    Bunthi, Charatdao
    Wonodi, Chizoba B.
    Morpeth, Susan C.
    Molyneux, Catherine S.
    Zaki, Sherif R.
    Levine, Orin S.
    Murdoch, David R.
    Scott, J. Anthony G.
    CLINICAL INFECTIOUS DISEASES, 2012, 54 : S165 - S171
  • [6] Etiology and management evaluation of community acquired pneumonia
    Torres, A
    Soler, N
    MEDICINA CLINICA, 2001, 116 (05): : 179 - 181
  • [7] Etiology of neonatal cholestasis after emerging molecular diagnostics
    Wang, Huanhuan
    Yang, Lin
    Wang, Jin
    TRANSLATIONAL PEDIATRICS, 2022, 11 (03) : 359 - +
  • [8] Mesotheliomas - current status in etiology, diagnostics, and molecular pathology
    Tannapfel, Andrea
    Tischoff, Iris
    PATHOLOGIE, 2024, 45 (05): : 303 - 304
  • [9] STUDIES ON MOLECULAR ETIOLOGY OF UREMIA
    GOTCH, FA
    SARGENT, JA
    PETERS, JH
    KIDNEY INTERNATIONAL, 1975, 7 (02) : S276 - S279
  • [10] Molecular diagnostics in the evaluation of thyroid nodules: Current use and prospective opportunities
    Patel, Jena
    Klopper, Joshua
    Cottrill, Elizabeth E. E.
    FRONTIERS IN ENDOCRINOLOGY, 2023, 14