Secretion of human interferon-β 1b by recombinant Lactococcus lactis

被引：10
|
作者：
Zhuang, Zhong [1 ]
Wu, Zhi-gang [1 ]
Chen, Min [1 ]
Wang, Peng George [1 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 10期
基金：
中国国家自然科学基金;
关键词：
human interferon beta; Lactococcus lactis; secretion; ulcerative colitis;
D O I：
10.1007/s10529-008-9761-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Interferon-beta has anti-viral, anti-proliferation and multifunctional immunomodulatory activities and shows promising clinical effects for treatment of inflammatory disorders. The recombinant human interferon-beta (huIFN-beta) 1b was expressed in the food-grade lactic acid bacterium, Lactococcus lactis, using a nisin-controlled gene expression system. huIFN-beta production from recombinant strains (with and without LEISSTCDA propeptide) was approximately 21 and 7 mu g l(-1), respectively. Moreover, 95% (former strain) and 88% (latter strain) of total recombinant proteins were secreted into the culture medium. The biological activities of huIFN-beta from recombinant strains revealed similar antiviral activities of 10(7) I.U. mg(-1). These results demonstrate the potential application of recombinant strains as a food grade vehicle to deliver bioactive huIFN-beta in vivo.
引用
收藏
页码：1819 / 1823
页数：5
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