Characterization of transgene loci in plants using FISH: A picture is worth a 1000 words

被引：16

作者：

Svitashev, SK ^{[1
]}

Somers, DA ^{[1
]}

机构：

[1] Univ Minnesota, Dept Agron & Plant Genet, Inst Plant Mol Genet, St Paul, MN 55108 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2002年 / 69卷 / 03期

关键词：

Agrobacterium tumefaciens; genetic engineering; microprojectile bombardment; plant transformation; transgene locus structure;

D O I：

10.1023/A:1015685501358

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic plants are traditionally characterized using phenotypic and Southern analyses. Over the last several years, fluorescence in situ hybridization (FISH) has been efficiently used for visualization, chromosomal localization and estimation of numbers of transgene loci in transgenic plants. Recent results obtained in different plant species transformed using either Agrobacterium tumefaciens or microprojectile bombardment indicate that FISH is also a powerful tool for characterization of transgene locus structure that significantly augments conventional Southern analysis. The aim of this review is to document the usefulness of FISH for characterization of transgene loci in plants.

引用

页码：205 / 214

页数：10

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