Real-Time PCR

被引：13
|
作者：
Jia, Yibing [1 ]
机构：
[1] OHSU, Oregon Natl Primate Res Ctr, Beaverton, OR USA
来源：
LABORATORY METHODS IN CELL BIOLOGY: BIOCHEMISTRY AND CELL CULTURE | 2012年 / 112卷
关键词：
POLYMERASE-CHAIN-REACTION; QUANTITATIVE PCR; RT-PCR;
D O I：
10.1016/B978-0-12-405914-6.00003-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Realtime PCR (or qPCR) is a widely used gold of standard for quantitative analysis of gene expression. It is also a very sensitive method for viral copy number detection, and genotyping. This Protocol outlines qPCR theory, data analysis and detailed procedures.
引用
收藏
页码：55 / 68
页数：14
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