Intracellular interleukin-1 receptor antagonist type 1 antagonizes the stimulatory effect of interleukin-1α precursor on cell motility

Interleukin (IL)-1 alpha, a proinflammatory cytokine, is produced as a 33 kDa protein precursor (preIL-1 alpha) which is cleaved to generate the 17 kDa C-terminal mature IL--1 alpha (mIL-1 alpha) and the 16 kDa N-terminal IL-1 alpha propiece (NIL-1 alpha). The biological effect of IL-1 alpha is regulated by the IL-1 receptor antagonist (IL-1Ra), its naturally occurring inhibitor. Four different isoforms of the IL-1Ra have been described, one secreted (sIL-1Ra) and three intracellular (icIL-1Ral1, 2, 3). Whether the icIL-1Ra1 isoform can antagonize some of the biological effects of intracellular IL-1 alpha is still unknown. The aim of this study is to investigate effects of preIL-1 alpha and icIL-1Ra1 on cell motility in stably transfected ECV304 cells. We show that expression of prelL-1 alpha in ECV304 cells significantly increases cell motility. Furthermore, transfection with NIL-la propiece also increases cell motility whereas this stimulatory effect was not observed by addition of exogenous mIL-1 alpha, suggesting an intracellular effect of preIL-1 alpha mediated by NIL-1 alpha propiece. Co-transfection of ECV304 cells with icIL-1Ra1 completely antagonizes the stimulatory effect of preIL-1 alpha and NIL-1 alpha propiece on cell motility. In conclusion, NIL-1 alpha propiece increases ECV304 cell motility and icIL-1Ra1 exerts intracellular functions regulating this stimulatory effect. (c) 2005 Elsevier Ltd. All rights reserved.