VirE1-mediated resistance to crown gall in transgenic Arabidopsis thaliana

被引:4
|
作者
Humann, J [1 ]
Andrews, S [1 ]
Ream, W [1 ]
机构
[1] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA
关键词
D O I
10.1094/PHYTO-96-0105
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Crown gall disease, caused by Agrobacterium tumefaciens, remains a serious agricultural problem despite current biocontrol methods. Agrobacterium tumefaciens transfers single-stranded DNA (T-strands) into plant cells along with several virulence proteins, including a: single-stranded DNA-binding protein (VirE2). In plant cells, T-strands are protected from nucleases and targeted to the nucleus by VirE2, which is essential for efficient transmission (transfer and integration) of T-strands. VirE1 is the secretory chaperone for VirE2; it prevents VirE2 from forming aggregates and from binding the T-strands in bacteria] cells. Therefore, we hypothesized that sufficient quantities of VirE1 expressed in plant cells might block T-DNA transmission by preventing VirE2 from binding T-strands. Here we show that root explants from Arabidopsis thaliana plants that expressed virE1 formed 3.5-fold fewer tumors than roots from plants without virE1. Also, this resistance was specific for VirE2-mediated Agrobacterium transformation. Plants that have been genetically altered to resist crown gall may prove more effective than biological control.
引用
收藏
页码:105 / 110
页数:6
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