Inflammation decreases the level of alpha7 nicotinic acetylcholine receptors in the brain mitochondria and makes them more susceptible to apoptosis induction

alpha 7 nicotinic acetylcholine receptors (alpha 7 nAChRs) are involved in regulating inflammatory reactions, as well as the cell viability. They are expressed in both the plasma membrane and mitochondria of eukaryotic cells. Previously we found that neuroinflammation resulted in the decrease of alpha 7 nAChR density in the brain of mice and was accompanied by accumulation of amyloid-beta (A beta) peptides and memory impairment In the present paper, it is shown that inflammation induced by either regular bacterial lipopolysaccharide (LPS) injections or immunizations with alpha 7 nAChR extracellular domain (1-208) affected also the brain cell mitochondria Using various modifications of sandwich ELISA, we observed the decrease of alpha 7 nAChRs and accumulation of A beta(1-40) and A beta(1-42) in mitochondria of immunized or LPS-treated mice compared to control ones. Mitochondria of treated mice responded with cytochrome c release to lower Ca2+ concentrations than mitochondria of control mice and were less sensitive to its attenuation with alpha 7 nAChR agonist PNU282987. It is concluded that inflammation decreases alpha 7 nAChR expression in both mitochondria and cell plasma membrane and makes mitochondria more susceptible to apoptosis induction. (C) 2015 Elsevier B.V. All rights reserved.