A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach

被引：2
|
作者：
Alves-Costa, Fernanda A. [1 ]
Figueiredo, Marcio A. [2 ]
Lanes, Carlos F. C. [2 ]
Almeida, Daniela V. [2 ]
Marins, Luis F. [2 ]
Wasko, Adriane P. [1 ]
机构：
[1] UNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP, Brazil
[2] Univ Fed Rio Grande FURG, Inst Ciencias Biol, BR-96201900 Rio Grande, RS, Brazil
来源：
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2012年 / 84卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
differential display; GH; growth hormone; transgenic; zebrafish; CYCLASE-ACTIVATING POLYPEPTIDE; GROWTH-HORMONE RELEASE; METABOLIC-RATE; P300; FISH; SECRETION; ALIGNMENT; SEQUENCE; ALTERS; PACAP;
D O I：
10.1590/S0001-37652012005000033
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A OH transgenic zebrafish (Danio rerio) line was compared to non-transgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in OH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 +/- 57.71; 224.10 +/- 91.73) when compared to NT samples (249.75 +/- 30.08; 342.95 +/- 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes.
引用
收藏
页码：487 / 493
页数：7
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