Expression of DNA Methyltransferase Genes in Four-Cell Bovine Embryos Cultured in the Presence of Oviductal Fluid

被引：9

作者：

Barrera, D. ^{[1
]}

Garcia, E. V. ^{[1
]}

Sinowatz, F. ^{[2
]}

Palma, G. A. ^{[3
]}

Jimenez-Diaz, M. A. ^{[1
]}

Miceli, D. C. ^{[1
]}

机构：

[1] Inst Super Invest Biol INSIBIO, San Miguel De Tucuman, Argentina

[2] Univ Munich, Inst Vet Anat Histol & Embryol, D-80539 Munich, Germany

[3] CITSE UNSE, RA-4200 El Zanjon, Santiago Del Es, Argentina

来源：

ANATOMIA HISTOLOGIA EMBRYOLOGIA | 2013年 / 42卷 / 04期

关键词：

IN-VITRO; PATTERN;

D O I：

10.1111/ahe.12010

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.

引用

页码：312 / 315

页数：4

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