Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains

被引:4
|
作者
Zokaeifar, Hadi [1 ]
Babaei, Nahid [1 ,2 ]
Saad, Che Roos [3 ]
Kamarudin, Mohd Salleh [3 ]
Sijam, Kamaruzaman [4 ]
Balcazar, Jose Luis [5 ]
机构
[1] Islamic Azad Univ, Sci & Res Branch Bushehr, Bushehr, Iran
[2] Islamic Azad Univ, Bushehr Branch, Bushehr, Iran
[3] Univ Putra Malaysia, Fac Agr, Dept Aquaculture, Serdang, Malaysia
[4] Univ Putra Malaysia, Fac Agr, Dept Plant Protect, Serdang, Malaysia
[5] Univ Girona, Catalan Inst Water Res ICRA, Girona, Spain
关键词
Bacillus subtilis; antibiotic biosynthesis genes; growth inhibition; Vibrio harveyi; SHRIMP; BACILYSIN; PROBIOTICS; ENZYME;
D O I
10.1080/09583157.2013.852653
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two Bacillus subtilis strains L10 and G1, previously isolated from fermented pickles, were tested for antibacterial activity against Vibrio harveyi using a well-diffusion agar assay. Antibiotic biosynthesis genes were then detected in both bacterial strains and included the following genes: bacA, bacB and bacD genes for bacilysin production, ppsE/fenB gene for plipastatin/fengycin production, albF and albA genes for subtilosin production, and srfAB and sfP genes for surfactin production. Based on these results, two B. subtilis strains could be considered as potential biological control agents in aquaculture.
引用
收藏
页码:233 / 240
页数:8
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