Chemical Synthesis of the 12 kDa Human Myokine Irisin by α-Ketoacid-Hydroxylamine (KAHA) Ligation

被引：8
|
作者：
Wucherpfennig, Thomas G. [1 ]
Mueller, Sebastian [2 ]
Wolfrum, Christian [2 ]
Bode, Jeffrey W. [1 ,3 ]
机构：
[1] Swiss Fed Inst Technol, Dept Chem & Appl Biosci, Lab Organ Chem, CH-8093 Zurich, Switzerland
[2] Swiss Fed Inst Technol, Dept Hlth Sci & Technol, Lab Translat Nutr Biol, CH-8603 Schwerzenbach, Switzerland
[3] Nagoya Univ, Inst Transformat Biomol WPI ITbM, Chikusa Ku, Nagoya, Aichi 4648602, Japan
来源：
HELVETICA CHIMICA ACTA | 2016年 / 99卷 / 12期
基金：
瑞士国家科学基金会;
关键词：
Proteins; Cytokines; Total synthesis; Metabolism; Ligation Reactions; PROTEIN-SYNTHESIS; 5-OXAPROLINE; PEPTIDES; GENE; FAT;
D O I：
10.1002/hlca.201600160
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Irisin is a recently discovered protein hormone with a conserved sequence among vertebrates and with putative functions in the regulation of adipose tissue and bone metabolism. We report the first chemical synthesis using two sequential ketoacid-hydroxylamine (KAHA) ligations to give milligram quantities of unlabeled and fluorescence-labeled irisin protein. The synthetic proteins were utilized in cell binding assays, which indicated the expected binding characteristics to stromal cells of white adipose tissue. These studies strongly imply the presence of a specific irisin receptor and provide a path to its identification with synthetic irisin.
引用
收藏
页码：897 / 907
页数：11
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