Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry

被引：82
|
作者：
Canene-Adams, Kirstie [1 ]
机构：
[1] Johns Hopkins Sch Med, Dept Pathol, Baltimore, MD USA
来源：
LABORATORY METHODS IN ENZYMOLOGY: CELL, LIPID AND CARBOHYDRATE | 2013年 / 533卷
关键词：
D O I：
10.1016/B978-0-12-420067-8.00015-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').
引用
收藏
页码：225 / 233
页数：9
相关论文
共 50 条
  • [1] Detection of Adenovirus in Formalin-Fixed Paraffin-Embedded Tissue by qPCR Compared to Immunohistochemistry
    Chang-Graham, Alexandra
    Pepper, Michael
    Sahoo, Malaya
    Huang, ChunHong
    Garamani, Natasha
    Higgins, John
    Pinsky, Benjamin
    [J]. LABORATORY INVESTIGATION, 2023, 103 (03) : S1276 - S1276
  • [2] P53 OVEREXPRESSION IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE DETECTED BY IMMUNOHISTOCHEMISTRY
    KERNS, BJM
    JORDAN, PA
    MOORE, MBH
    HUMPHREY, PA
    BERCHUCK, A
    KOHLER, MF
    BAST, RC
    IGLEHART, JD
    MARKS, JR
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (07) : 1047 - 1051
  • [3] Utility of Immunohistochemistry for Tissue Transglutaminase in the Diagnosis of Celiac Disease on Formalin-Fixed, Paraffin-Embedded Tissue
    Teo, I.
    Marginean, H.
    Nguyen, B.
    Marginean, E. C.
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2010, 134 (04) : 679 - 679
  • [4] Effects of Preanalytical Variables on the Detection of Proteins by Immunohistochemistry in Formalin-Fixed, Paraffin-Embedded Tissue
    Engel, Kelly B.
    Moore, Helen M.
    [J]. ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2011, 135 (05) : 537 - 543
  • [5] A RAPID NISSL STAIN FOR FORMALIN-FIXED PARAFFIN-EMBEDDED TISSUE
    PUTT, FA
    [J]. JOURNAL OF NEUROSURGERY, 1948, 5 (02) : 211 - 211
  • [6] Protein expression analysis in formalin-fixed, paraffin-embedded tissue
    Steinert, K.
    Becker, K. -F.
    Schott, C.
    Erpenbach, A.
    Porschewski, P.
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (10) : S167 - S167
  • [7] Profiling of lymphoma from formalin-fixed paraffin-embedded tissue
    Robetorye, Ryan S.
    Maguire, Alanna
    Rosenthal, Alison C.
    Rimsza, Lisa M.
    [J]. SEMINARS IN HEMATOLOGY, 2019, 56 (01) : 46 - 51
  • [8] Fluorescence in situ hybridization on formalin-fixed and paraffin-embedded tissue
    Petersen, BL
    Sorensen, MC
    Pedersen, S
    Rasmussen, M
    [J]. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2004, 12 (03) : 259 - 265
  • [9] Massively parallel sequencing of formalin-fixed, paraffin-embedded tissue
    Eyerson, Richard B.
    Glatt-Deeley, Heather R.
    Burian, Linda C.
    Perpetua, Lorrie A.
    Hegde, Upendra P.
    Lambrecht, Richard W.
    Sanders, Mary M.
    [J]. CANCER RESEARCH, 2011, 71
  • [10] PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA
    Nicholson E.M.
    Greenlee J.J.
    Hamir A.N.
    [J]. BMC Research Notes, 4 (1)
12345