Enhanced production of streptomycin and hydrolytic enzymes by Streptomyces griseus strains using different types of organic solvents and detergent compounds

Different organic solvents and detergents showed profound effects on the production of streptomycin (SM), amylases and proteases by Streptomyces griseus strains. The maximum SM production (1128 and 780 mg/L) was Obtained using Streptomyces NRRL-strain when ethanol (1.0% v/v) and Tween-80 (0.1%v/v) were added to the medium. Production of amylases was also enhanced using the strain with the addition of benzene at decline phase, n-butanol at the trophophase and isopropanol at the idiophase with values, 10.66, 8.28 and 8.05 U/mL for alpha-amylase (EC-3.2.1.1) and 6.28, 4.68 and 4.55 for beta-amylase (EC-3.2.1.2), respectively. While the use of other solvents and detergents reduced the production of amylase specially activities by S. griseus DSM-40759, characterized by higher productions of alpha and beta-amylases in different growth phases especially at the death phase. Worthily, proteases secretion was only induced to 1.02 U/mL at the idiophase by culturing DSM-strain with the addition of Triton X-100 and SDS to the fermentation medium. Moreover, the solvents shifted the pH values in the acidic range by the two strains at different phases compared to the control.