Glucose amperometric biosensor based on the co-immobilization of glucose oxidase (GOx) and ferrocene in poly(pyrrole) generated from ethanol/water mixtures

被引：0
|
作者：
Fiorito, PA [1 ]
de Torresi, SIC [1 ]
机构：
[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, Brazil
来源：
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2001年 / 12卷 / 06期
关键词：
amperometric biosensor; polypyrrol; glucose detection; ferrocene mediation;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The co-immobilization of GOx and ferrocene in a polymeric matrix by a one-step simple method is presented. This procedure to immobilize ferrocene as mediator implies the absence of modification of the monomer or the enzyme that would lead to the loss of its activity. Ferrocene incorporation results in an increase of sensitivity compared with the sensor prepared without the redox mediator (1.5 vs 0.23 muA mmol(-1) L cm(-2)) and the decrease of the working potential to 0.4 V. The prepared sensor shows linear response till 10 mmol L-1 and response time of 2 s, in addition to reasonable stability after one week.
引用
收藏
页码：729 / 733
页数：5
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