Silica/Titania Graphite Composite Modified with Chitosan and Tyrosinase Employed as a Sensitive Biosensor for Phenolic Compounds

A mesoporous, high surface area and conductive silica/titania graphite composite modified with chitosan and functionalized with tyrosinase was prepared. The composite was obtained by the sol-gel method and the surface of the resultant material was modified with chitosan solutions containing 5, 10 and 15% by mass. The textural characterization showed that even after modification with chitosan, the composite maintains a pore volume near 0.70 cm(3) g(-1) and pore diameter between 10 and 50 nm. adequate for enzyme immobilization. The tyrosinase was immobilized on the composite that was modified with 5% chitosan solution. A carbon ceramic electrode was manufactured with this material and was used as an electrochemical biosensor for phenolic compounds, showing low detection limits in the linear range from 40 to 350 mu mol L-1 and good sensitivity of 0.284, 0.141 and 0.165 mu A mu mol(-1)L for catechol. dopamine and pyrogallol, respectively. In this composite the enzyme showed to be operative and stable after several months.