Characterization of the α-haemolysin determinant from the human enteropathogenic Escherichia coli O26 plasmid pEO5

被引:8
|
作者
Burgos, Ylanna Kelner [1 ,2 ]
Pries, Karin [1 ]
Pestana de Castro, Antonio Fernando [2 ]
Beutin, Lothar [1 ]
机构
[1] BfR Fed Inst Risk Assessment, Natl Reference Lab Escherichia Coli, D-12277 Berlin, Germany
[2] Univ Sao Paulo, Inst Ciencias Biomed 2, Dept Microbiol, Sao Paulo, Brazil
关键词
Escherichia coli; EPEC; EHEC; O26; alpha-haemolysin; virulence plasmid; LARGE VIRULENCE PLASMID; NUCLEOTIDE-SEQUENCE; CHROMOSOMAL HEMOLYSIN; ENCODED HEMOLYSIN; WEANED PIGS; STRAINS; OPERON; GENES; SECRETION; MECHANISM;
D O I
10.1111/j.1574-6968.2009.01496.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The 157-kb conjugative plasmid pEO5 encoding alpha-haemolysin in strains of human enteropathogenic Escherichia coli (EPEC) O26 was investigated for its relationship with EHEC-haemolysin-encoding plasmids of enterohaemorrhagic E. coli (EHEC) O26 and O157 strains. Plasmid pEO5 was found to be compatible with EHEC-virulence plasmids and did not hybridize in Southern blots with plasmid pO157 from the EHEC O157:H7 strain EDL933, indicating that both plasmids were unrelated. A 9227-bp stretch of pEO5 DNA encompassing the entire alpha-hlyCABD operon was sequenced and compared for similarity to plasmid and chromosomally inherited alpha-hly determinants. The alpha-hly determinant of pEO5 (7252 bp) and its upstream region was most similar to corresponding sequences of the murine E. coli alpha-hly plasmid pHly152, in particular, the structural alpha-hlyCABD genes (99.2% identity) and the regulatory hlyR regions (98.8% identity). pEO5 and alpha-hly plasmids of EPEC O26 strains from humans and cattle were very similar for the regions encompassing the structural alpha-hlyCABD genes. The major difference found between the hly regions of pHly152 and pEO5 is caused by the insertion of an IS2 element upstream of the hlyC gene in pHly152. The presence of transposon-like structures at both ends of the alpha-hly sequence indicates that this pEO5 virulence factor was probably acquired by horizontal gene transfer.
引用
收藏
页码:194 / 202
页数:9
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