STUDIES ON BIOMETRY OF SPERM OF MURRAH BUFFALO BULLS (BUBALUS BUBALIS)

Objective of the present study was to measure various biometric parameters of the spermatozoa of Murrah buffalo bulls. Spermatozoa with intact acrosomes were selected and assessed using an immersion lens (1,000X) and standard illumination. The software made it possible to take linear measurements of each spermatozoon: head length, head width, head base, tail length, acrosomal cap length and acrosomal cap width. The results of the morphometric study did not demonstrate the existence of sperm subpopulations. Mean head length, mean head width, mean head base, mean acrosomal cap length and mean acrosomal cap width were 7.59 +/- 0.01 mu m, 4.91 +/- 0.01 mu m, 2.47 +/- 0.01 mu m, 3.46 +/- 0.01 mu m and 4.51 +/- 0.01 mu m, respectively. The mean tail length was 56.14 +/- 0.01 mu m.