Lysinibacillus mangiferahumi sp. nov., a new bacterium producing nematicidal volatiles

A Gram-positive bacterium, designated M-GX18(T), was isolated from the rhizosphere soil of mango (Guangxi Province, China). The isolate produced nematicidal volatile compounds with activities against the root-knot nematode . The organism was motile, catalase- and oxidase-positive, spore-forming and rod shaped. The predominant menaquinone was MK-7 and the major cellular fatty acid profiles were iso-C-15:0, anteiso-C-15:0 and iso-C-16:0. The DNA G+C content was 38.9%. A phylogenetic analysis based on 16S rRNA gene sequences showed that this organism represented a new species of the genus . Strain M-GX18(T) exhibited high 16S rRNA gene sequence similarity with its closest neighbors (98.5%), (98.1%) and (98.6%). The physiological, biochemical and chemotaxonomic data, including DNA-DNA hybridization relatedness data, indicate that strain M-GX18(T) can be distinguished from all the related species of the genus . Therefore, on the basis of the polyphasic taxonomic data presented, a new species of the genus , , with the type strain M-GX18(T) (=DSM 24076(T) = CCTCC AB 2010389(T)) is proposed.