Temperature-jump apparatus with Raman detection based on a solid-state tunable (1.80-2.05 μm) kHz optical parametric oscillator laser

被引：18

作者：

Balakrishnan, G ^{[1
]}

Hu, Y ^{[1
]}

Spiro, TG ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

APPLIED SPECTROSCOPY | 2006年 / 60卷 / 04期

关键词：

optical parametric oscillator; near-infrared laser; temperature jump; protein folding; ultraviolet resonance Raman; cytochrome c;

D O I：

10.1366/000370206776593799

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (similar to 10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing similar to 1 mJ NIR pulses that are tunable (1.80-2.05 mu m) across the 1.9 mu m vibrational\ overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 degrees C, as measured via the O-H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.

引用

页码：347 / 351

页数：5