The versatility of human immunodeficiency virus (HIV)-1 and its evolutionary potential to elude antiretroviral agents by mutating may be its most invincible weapon. Viruses, including HIV, in order to adapt and survive in their environment evolve at extremely fast rates. Given that conventional approaches which have been applied against HIV have failed, novel and more promising approaches must be employed. Recent studies advocate RNA interference (RNAi) as a promising therapeutic tool against HIV. In this regard, targeting multiple HIV sites in the context of a combinatorial RNAi-based approach may efficiently stop viral propagation at an early stage. Moreover, large high-throughput RNAi screens are widely used in the fields of drug development and reverse genetics. Computer-based algorithms, bioinformatics, and biostatistical approaches have been employed in traditional medicinal chemistry discovery protocols for low molecular weight compounds. However, the diversity and complexity of RNAi screens cannot be efficiently addressed by these outdated approaches. Herein, a series of novel workflows for both wet-and dry-lab strategies are presented in an effort to provide an updated review of state-of-the-art RNAi technologies, which may enable adequate progress in the fight against the HIV-1 virus.
机构:
Univ Amsterdam, Dept Human Retrovirol, Acad Med Ctr, NL-1105 AZ Amsterdam, NetherlandsUniv Amsterdam, Dept Human Retrovirol, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands
机构:
Singapore Polytechn, Sch Chem & Life Sci, Singapore, SingaporeSwinburne Univ Technol, Fac Life & Social Sci, Hawthorn, Vic 3122, Australia
Tan, Eng Lee
Marcus, Kah Fai Ho
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Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol, Singapore 117595, SingaporeSwinburne Univ Technol, Fac Life & Social Sci, Hawthorn, Vic 3122, Australia
Marcus, Kah Fai Ho
Poh, Chit Laa
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Swinburne Univ Technol, Fac Life & Social Sci, Hawthorn, Vic 3122, AustraliaSwinburne Univ Technol, Fac Life & Social Sci, Hawthorn, Vic 3122, Australia