Limits to Resolution of CW STED Microscopy

被引：6
|
作者：
Trifonov, Alexei S. [1 ,2 ,3 ]
Jaskula, Jean-Christophe [2 ]
Teulon, Claire [1 ]
Glenn, David R. [2 ]
Bar-Gill, Nir [1 ]
Walsworth, Ronald L. [1 ,2 ]
机构：
[1] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
[2] Harvard Smithsonian Ctr Astrophys, Cambridge, MA 02138 USA
[3] RAS, Ioffe Phys Tech Inst, St Petersburg, Russia
来源：
ADVANCES IN ATOMIC, MOLECULAR, AND OPTICAL PHYSICS, VOL 62 | 2013年 / 62卷
关键词：
NITROGEN-VACANCY CENTERS; STIMULATED-EMISSION; DEPLETION MICROSCOPY; DIAMOND;
D O I：
10.1016/B978-0-12-408090-4.00005-0
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CVV) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED "doughnut" beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.
引用
收藏
页码：279 / 302
页数：24
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