Development and validation of a Myxoma virus real-time polymerase chain reaction assay

被引：8

作者：

Albini, Sarah ^{[1
]}

Sigrist, Brigitte ^{[1
]}

Guettinger, Regula ^{[1
]}

Schelling, Claude ^{[2
,3
]}

Hoop, Richard K. ^{[1
]}

Voegtlin, Andrea ^{[1
]}

机构：

[1] Univ Zurich, Inst Vet Bacteriol, Natl Reference Ctr Poultry & Rabbit Dis, Vetsuisse Fac, CH-8057 Zurich, Switzerland

[2] Swiss Fed Inst Technol, Vetsuisse Fac Zurich, Anim Genet Grp, Zurich, Switzerland

[3] Swiss Fed Inst Technol, Dept Anim Sci, Zurich, Switzerland

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2012年 / 24卷 / 01期

关键词：

Myxomatosis; Myxoma virus; rabbits; real-time polymerase chain reaction assay; EUROPEAN RABBIT; AUSTRALIA;

D O I：

10.1177/1040638711425946

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus in fresh and paraffin-embedded rabbit tissues originating from myxomatosis cases from various regions in Switzerland.

引用

页码：135 / 137

页数：3

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